Dataset Detail

Dataset: Prevalence of Infection in Tubifex tubifex Under Different Flow Rates


Project: Assessment of the risk of Myxobolus cerebralis Introduction as a Result of Straying Adult Steelhead and Spring Chinook Salmon in the Columbia River Basin

Dataset Abstract: Myxospores were introduced to artificial stream channels with different flow regimes (slow and fast) and containing Tubifex tubifex. Worms were removed 201 days post-exposure and a quantitative PCR (QPCR) assay was performed for Myxobolus cerebralis. Data show the results of the QPCR, including raw Ct values for those worms that tested positive and a summary of adjusted raw data. Ct values indicate severity of infection and are inversely proportional to the amount of parasite DNA found in each worm.
Significance of Results: The percentage of Tubifex tubifex that tested positive by QPCR for Myxobolus cerebralis was affected by flow rate.
Relevance to Managers: The larger study demonstrates that water flow rate (even a 100 fold difference) has a significant effect on the propagation of M. cerebralis and its impact on its fish host. Thus, in a system where water release is managed by dams or draw-off for agriculture or other uses, flow can be used to reduce effects of the parasite. This study suggests that even small increases in flow in critical low-flow habitats can significantly reduce infection in both hosts (fish and worms).
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